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KMID : 0900919840080010010
Korean journal of Animal Reproduction
1984 Volume.8 No. 1 p.10 ~ p.15
The Study of Egg Transports and distribution Patterns in the Oviducts of Superovulated Rabbits by Administrations of PGF_{2alpha}


Shim Keum-Seob
Abstract
This experiment was carried out to study the egg transports and distribution patterns in the oviducts of superovulated rabbits by administrations of PGF2alpha. Group I (3 does) and Group II (3 does) were killed at 24 hours, 48 hours after mating, respectively. Group III (6 does0 received a single injection of 5mg/Kg of body weight of PGF2alpha at 24 hours after mating and killed at 4 hours after PGF2alpha administration. The oviducts were frozen in LN-gas, thawed using Howe¡¯s method, and cleared in Benzyl-Benzoate sol. using Orsini¡¯s technique. The location of the eggs and the AIJ. were identified using transmitted light from a dissecting microscope. The results obtained were as follows: 1. The average numbers of detected eggs from the oviduct were 28.6 (26-32), 26.0 (24-29), and 27.1 (22-34) in Group I, II, and III, respectively. 2. Detection rate was 88.7%, 76.4%, and 84.9% in Group I, II, and III, respectively. 3. The positionof all AIJ. was 44.2% to 55.0%(av. 49.5%) of the distance from the fimbriae to the UTJ.(Utero-Tubal Junction). 4. The changes of egg distribution patterns were as follows: Group I Ampulla : 52.3%, Isthmus : 47.7%, Group II Ampulla : 8.9%, Isthmus : 91.1% Group III Ampulla : 8.0% Isthmus : 92.0%.
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